PVP K30 Polyvinylpyrrolidone Luvitec K30

PVP K-30 can be used as enzyme stabilizer. Encapsulation - sustained-release agent; Adhesive, stabilizer, protective colloid, film forming agent.

PVP is easily soluble in water, alcohol, amine and halogenated hydrocarbons, but insoluble in acetone, ether and so on. It has excellent solubility, biological compatibility, physiological inertness, film forming, membrane protection ability and the ability to compound with a variety of organic and inorganic compounds, and is stable to acid, salt and heat, so it has a wide range of uses. Used to absorb phenols and tannins from water extracts to purify plant enzymes. Used as a chromatographic adsorbent to separate aromatic acids, aldehydes and phenols. Used for clarification of beer and wine.

Action mechanism

Stabilizing and protective effects

Enzyme/antibody stabilizer: Through hydrogen bonding and hydrophobic interactions, the coating of biological macromolecules (such as enzymes, antibodies) prevents their denaturation or aggregation, extending the preservation time of activity.

Protective colloid: inhibit the aggregation and precipitation of particles (such as latex microspheres and gold nanoparticles) to maintain the uniformity of the detection system.

Dispersion and solubilization

Dispersant: Through steric hindrance effect to prevent the aggregation of hydrophobic particles (such as lipids, insoluble substrates), improve the reaction efficiency.

Solubilization: Increases the solubility of hydrophobic substances (such as certain chemiluminescent substrates) in the aqueous phase.

Film formation and slow release

Film formation: A uniform film is formed on the surface of the microplate and the test strip to protect the active ingredient from environmental interference (such as oxidation).

Slow-release carrier: through physical embedding or adsorption, control the release rate of detection reagents (such as POCT test strip color development time regulation).

Application scenario

immunoassay

ELISA/ chemiluminescence: Used as a blocking or diluent component to reduce non-specific adsorption (such as closing unbound sites on the surface of microplates).

Latex enhanced immune turbidimetry: Stabilizes latex particle-antibody complexes to prevent false positives/false negatives caused by aggregation.

Molecular diagnosis

PCR/qPCR: Inhibit non-specific binding of DNA polymerase to improve amplification specificity.

Nucleic acid extraction: adsorption of phenols, tannic acid and other interferences in the sample, purification of DNA/RNA (similar to the purification principle of plant enzymes).

Biochemical detection

Enzymatic reaction system: stabilizes enzyme activity (such as horseradish peroxidase HRP) to prevent substrate autoxidation.

Formulation of developing substrate: dispersing insoluble developing agent (such as TMB) to ensure uniformity of developing color.

Point-of-care Detection (POCT)

Strip coating: Improve the hydrophilicity of nitrocellulose film, accelerate sample diffusion and shorten detection time.

Usage amount

Conventional concentration range

Sealer/diluent: 0.1%-1% (such as 0.5% PVP K30 in ELISA).

Particle dispersion system: 0.5%-3% (such as latex particle suspension).

Enzyme stabilizer: 0.1-0.5% (need to verify the effect on enzyme activity).

Nucleic acid purification: 1%-5% (adsorption of phenolic interferences).

Dissolution method

Direct dissolution: easily soluble in water, ethanol, stirring at room temperature can be dissolved without heating.

Premixed storage: It is recommended to prepare 10% reserve solution to avoid long-term storage to breed microorganisms.

Matters needing attention

Sealed and moisture-proof store in a cool and dry place (15-25 ° C recommended) to avoid moisture absorption agglomeration or microbial contamination.

Long-term storage may be slightly yellowed, does not affect function, but need to avoid light.

Temperature sensitivity: High temperature (>80℃) may lead to molecular chain breakage and loss of film formation.

pH compatibility: Stable in pH 3-10 range, may degrade under strong acid/base conditions.

Plastic containers: long-term contact may swell polystyrene (PS) containers, it is recommended to use short-term or use glass containers.

Protein interference: Excess may adsorb antigens/antibodies, and the concentration needs to be optimized through pre-experiments.

incompatibility

Strong oxidizing agent: Oxidation reaction may occur with high concentration persulfate, hypochlorite, etc.

Polyvalent cations: may form precipitates when combined with Al³ +, Fe³ +, etc.

Q&A

Q: Why is PVP K30 commonly used in ELISA sealers?

A: Its film-forming ability can cover the hydrophobic area on the surface of the microporous plate, reduce non-specific adsorption, and do not interfere with antibody-antigen binding.

Q: Is it normal for the solution to thicken after use?

A: Normal, PVP K30 is a polymer, and the viscosity of the system will be significantly increased when the concentration is greater than 1%, and the dosage should be adjusted according to the testing requirements.

Q: Can you replace BSA as a blocking agent?

A: Yes, but the blocking effect needs to be verified (PVP costs less, but may not be as effective as BSA for some high background systems).

Q: Does PVP K30 affect fluorescence detection?

A: Low concentration (<0.5%) has no significant interference, but high concentration may cause background elevation due to light scattering, and the concentration needs to be optimized.

Q: How to deal with incomplete dissolution of PVP K30?

A: Make sure to use pure water or buffer to avoid excessive ionic strength; Can be slightly heated (<40℃) to accelerate dissolution.

Luvitec  is a trademark of BASF