Protein desalting column(5KMWCO,6ml)

Protein desalination centrifuge column is a modified dextran desalination device with a packing particle size of 58-80 microns, which can achieve rapid and excellent desalination and high recovery for proteins larger than 5K&30K MWCO. Samples from 100 μ L to 2mL provide trouble-free desalination and buffer exchange.

»High recovery-low binding resin greatly improves protein recovery.

»Fast-No need to screen or wait for gravity-dependent proteins to flow out.

»Economy-more economical and practical than other pre-installed columns sold in the market.

»stable-reusable.

Operation steps.

1. Centrifuge to remove the storage liquid. 1000 × g centrifugal 2min was used to remove the storage liquid.

2. Balance preloaded columns. Add the buffer solution needed for 350 μ l sample and place 1000 × g centrifugal 2min for 2 min. Then add 200 μ l of the above buffer, repeat the above balance operation 2 times.

3. Sample treatment. Add the protein samples that need to be treated, place 30Smine1000 × g centrifugal 2min, collect and recover the samples, save them and set aside.

Method of reuse and preservation of desalting column.

The used desalting column was added to 200 μ l PBS solution containing 2m NaCl for 4 times, and 1000 × g centrifugal 2min was placed for 2 min. Then 200 μ l deionized water was added three times and 1 000 × g centrifugal 2min was placed for 2 minutes. The treated desalting column is added with 20% ethanol solution and can be preserved.

Matters needing attention.

1. The sample handling capacity should be within the range of column treatment, too much will lead to incomplete desalination, and too little will lead to a decrease in sample recovery.

2. The packing will lose water and shrink in high concentration alcohol solution or saturated salt solution. Do not pass the above solution through the column.

3. When adding samples, try to add them evenly to the center of the tube.

4. When the sample concentration is too low, the sample needs to be concentrated to the desired volume or concentration.

common problem.

1. The recovery rate of protein is low.

Analysis: a, protein concentration is too low (< 0.01mg/ml).

B, the composition of the replaced buffer or PH non-optimal buffer produces non-specific adsorption.

Treatment: a. Desalination after proper concentration of protein solution.

B. Replace the suitable buffer and fully balance the preloaded column 2-3 times.

2. Turbidity or precipitation occurs after protein recovery.

Analysis: a, buffer is not the most suitable buffer.

B. some ions in the buffer were removed, resulting in protein isoelectric point coalescence.

Treatment: replace the suitable buffer and fully balance the preloaded column 2-3 times.

3. Desalination is not clean.

Analysis: salt ion concentration is too high (> 0.5m).Protein desalting column

Treatment: properly increase desalination times or desalination after dilution according to salt ion concentration.

Recommended sample quantity: