Spectinomycin dihydrochloride pentahydrate

Application.
Spectinomycin is a kind of aminocyclohexol antibiotics derived from Streptomyces magnificent. It has been proved to have a wide range of uses, including the treatment of acute gonococcal urethritis and cervicitis, labeling cell layers to monitor cell fate during leaf development, and as a selective marker in the plant-related transformation system containing the tagged gene Spcr, to study bovine respiratory tract infections caused by Pasteurella multocida and Mansteurella haemolyticus. And plants lacking plastid coding RNA polymerase on MS Agar medium. Spectinomycin is also used to amplify replicators carrying low copy number plasmids. It is recommended for cell culture applications with a concentration of 7.5-20 mg/L.

Spectinomycin dihydrochloride pentahydrate has been used as a supplement to different types of culture media.

General description.
Chemical structure: aminoglycoside spectinomycin is commonly used to treat male gonorrhea, and a study has shown that spectinomycin dihydrochloride pentahydrate is also effective in the treatment of gonorrhea. Clinically, spectinomycin dihydrochloride pentahydrate has higher solubility than spectinomycin, so it is more convenient for injection.

Biochemical / physiological behavior.
Mechanism of action: spectinomycin inhibits protein synthesis and extension by binding to bacterial 30s ribosomal subunit and interfering with peptide tRNA translocation. Resistance mechanism: rpsE (ribosomal protein S5 gene) mutation can prevent spectinomycin binding. Aminoglycoside-3-adenosine transferase gene (aadA) can also make bacteria resistant. Antibacterial spectrum: spectinomycin can fight against Gram-negative and Gram-positive bacteria.

Warning.
The solution can be stored at 2-8 °C for several weeks or longer at-20 °C.

Points for attention in preparation.
When preparing the storage solution, 10 mg/mL should be dissolved in water and filtered and sterilized.

Other considerations.
Keep it in an airtight container and place it in a dry and ventilated place. Store in a dry place.