Nystatin

Warning.
The tissue culture medium solution of nystatin can be stably preserved at 37 °C for three days. It is also stable in moderately alkaline media. The solution and aqueous suspension begin to lose their activity soon after preparation, and heat, light and oxygen accelerate the decomposition. If heated to 100 °C at pH 7, the aqueous suspension can be stably preserved for 10 min.

Biochemical / physiological behavior.
Mechanism of action: nystatin acts by binding to ergosterol in fungal cell membrane, increasing membrane permeability and producing nystatin / ergosterol-based ion channels. It can also be used as a lipid raft inhibitor and membrane-associated cholesterol. Antimicrobial spectrum: nystatin can fight fungi, yeast and mold.

Points for attention in preparation.
At 28 °C, nystatin was soluble in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL) and ethylene glycol (8.75 mg/mL). It can also be 5 mg/mL soluble in DMSO and easily soluble in DMF and formamide. High pressure sterilization or aseptic filtration of nystatin solution is not recommended. Nystatin suspension is also effective and can be kept stably for 3 days at 37 °C. 50 mg/mL suspension in water was prepared and stored at-20 °C.

Application.
Nystatin is a Polyene macrolide antibiotic. Its uses include preventing cell culture contamination and inducing the secretion of interleukin (IL)-1, IL-8 and tumor necrosis factor-α in THP1 cells expressing TLR2. This product is recommended for cell culture with a concentration of 50 mg/L.