Mineral oil

Application.
It is recommended to cover mouse embryo culture to prevent dryness.

Analysis consideration.
The material was tested as a covering in a droplet embryo culture. (all embryo test materials were tested with mouse embryos. BCF1 × BDF1 heterozygote is usually used. The oil is mainly used to prevent the evaporation of the culture medium, especially when embryo operation is carried out. The process is as follows: 1-cell (BCF1 × BDF1) embryos were cultured in three microdroplets of embryo-tested HTF (human fallopian tube fluid) medium supplemented with 0.4% BSA. As an effective experiment, at least 70% of the 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of the 1-cell stage test embryos must develop to the blastocyst stage with test materials within 96 hours.

Warning.
The product is not aseptic. Mineral oil is difficult to sterilize. It can only be sterilized by carefully filtering warm oil. It is assumed that antibiotics will be added to the cell medium.