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Hygromycin B from Streptomyces hygroscopicus

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Description
Product Name Hygromycin B from Streptomyces hygroscopicus
Types S homologous reagent
Form powder
CAS NO 31282-04-9
Producer China
Source Streptomyces hygroscopicus
Application protein synthesis | interferes
Purity powder, BioReagent, suitable for cell culture
Storage 2-8°C
Recertification 4Years
Synonyms Hygromycin B solution - SG12894-10ML
Hygromycin B from Streptomyces hygroscopicus - SG12296-250MG
Overview
Biological source Streptomyces hygroscopicus
Content ≥60% (HPLC)
Form powder
Application cell culture | mammalian: suitable
Color faintly brown to brown, white to beige
Melting point 160-180 °C
Dissolubility H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
Mechanism protein synthesis | interferes
Antibacterial spectrum fungi
Storage 2-8°C

General description.
Chemical structure: aminoglycosides are the most widely used antibiotics targeting ribosomes. Hygromycin B is an aminocyclophenol antibiotic. Streptomyces hygroscopicus has phosphotransferase (HPH) activity, which can phosphorylate hygromycin B, which helps to activate autoimmunity against the toxicity of the drug.

Application.
Hygromycin B antibiotics can be used for the moisture resistance of the following cells: 160u g/ml for 293T cells transfected with plasmid mixture. 150 g/ml for MCF-7ALOX12 cells. MSCV luciferase PGK-hygro plasmid. STAT3 reporter gene HeLa stable cell line (stably transfected with luciferase gene controlled by STAT3 promoter) was maintained in DMEM supplemented with 10%FBS.

Biochemical / physiological behavior.
Mode of action: this product can inhibit translation by inducing misreading of m-RNA template in prokaryotes and inhibiting protein synthesis. Antibacterial spectrum: hygromycin B has inhibitory effect on bacteria, fungi and higher eukaryotic cells.

Warning.
The product of hygromycin B should be stored at a temperature of 2-8 °C, and its dry fixed form can be stored at a temperature of 2-8 °C for at least five years. It can be stable for 30 days at 37 °C. (for solution) if stored at 2-8 °C, the solution can remain stable for two years.

Points for attention in preparation.
The product was purified by ion exchange chromatography. Hygromycin B can be dissolved in water at a concentration of more than 50 mg/mL, or in methanol or ethanol. The solution should be sterilized by filtration rather than high pressure sterilization. In addition, it is reported that hygromycin B solution will lose its activity during freezing, and it can remain stable in cold storage, so freezing should be avoided.

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SKU UNIT PRICE (USD) QTY
SG32770-100MG 100MG 51.6
SG32770-1G 1G 187