DTT | DL-Dithiothreitol

Application.
DTT has been used in: 1. As one of the reactants of α S1-casein reduction and alkylation, it is the main allergen of milk. two。. As a medium for sperm detachment and reactivation. 3. Maintain the stability of sulfhydryl groups in the enzyme and effectively protect the active sites of the biocatalysts. 4. As a reducing agent, the specificity of the reaction of N-ethyl Maleimide with sulfhydryl group was tested.

An excellent reagent for keeping the-SH group in the reduced state; quantitative reduction of disulfides. Before SDS-PAGE, DTT could effectively reduce the disulfide bond of protein in the sample buffer. DTT can also be used to reduce the disulfide bond bridge of cross-linking agent Nomencline-bis (acryloyl) cystamine to decompose polyacrylamide gel matrix. The irritation and toxicity of DTT were lower than that of 2-mercaptoethanol. In general, the concentration required to use DTT (100mM) is 1/7 of the concentration using 2-mercaptoethanol (700mM).